Difference between revisions of "Acetobacter"

From Milk The Funk Wiki
Jump to: navigation, search
(added alcohol and glucose tolerance)
 
(11 intermediate revisions by the same user not shown)
Line 1: Line 1:
(In progress) First, define '''Acetobacter'''.
+
'''Acetobacter''' is a genus of obligatory aerobic bacteria that is in the family ''Actobacteraceae''.  This family is more commonly known as "acetic acid bacteria" (AAB).  Other genera of AAB include ''Gluconobacter'', ''Gluconacetobacter'', ''Acidomonas'', ''Asaia'', ''Kozakia'', ''Swaminathania'' and ''Saccharibacter'' <ref name="Gullo_2005" />.  
  
 
POTENTIAL REFERENCES:
 
POTENTIAL REFERENCES:
Line 9: Line 9:
  
 
https://www.facebook.com/groups/MilkTheFunk/permalink/1356322957729248/
 
https://www.facebook.com/groups/MilkTheFunk/permalink/1356322957729248/
 +
 +
https://www.homebrewersassociation.org/how-to-brew/sour-microbes-yeast-and-bacteria-explained/
 +
 +
Leads on fermentation: http://www.mdpi.com/2311-5637/4/3/66
  
 
[[Flanders Red Ale]]
 
[[Flanders Red Ale]]
  
 
==Metabolism==
 
==Metabolism==
This should be written similar to [[Lactobacillus#Metabolism]], and is science heavy but layman enough for brewers to understand.
+
Acetic acid is produced by acetic acid bacteria through the oxidation of carbon sources into ethanol, followed by the oxidation of ethanol into acetaldehyde, and finally through the oxidation of acetaldehyde into acetic acid. When ethanol is depleted, acetic acid bacteria can also convert glycerol into cellulose, acetic acid, and carbon dioxide. Acetic acid bacteria have also been linked to elevated levels of acetoin, a pleasant buttery flavor, as well as ethyl acetate <ref name="Bossaert_2019">[https://www.researchgate.net/publication/341353855_The_power_of_sour_-_A_review_Old_traditions_new_opportunities Bossaert, Sofie, et al. “The Power of Sour - A Review: Old Traditions, New Opportunities.” BrewingScience, vol. 72, no. 3-4, 2019, pp. 78–88.]</ref>.  
  
 
===Alcohol and Sugar Tolerance===
 
===Alcohol and Sugar Tolerance===
Tolerance to ethanol begins to decline in ''Acetobacter'' starting at about 10% ABV.  Most strains are not tolerant of glucose once it is at ~25% of the solution <ref name="Gullo_2005">[Characterization of acetic acid bacteria in “traditional balsamic vinegar”.  Maria Gullo, Cinzia Caggia, Luciana De Vero, Paolo Giudici.  2005.]</ref>.
+
Tolerance to ethanol begins to decline in most ''Acetobacter'' species and strains starting at about 10% ABV.  However, this is species and strain dependent.  For example, some strains of ''A. aceti'' and ''A. pasteurianus'' (80% of strains tested) are unable to grow in 10% ABV.  Other genera of acetic acid bacteria such as ''Gluconobacter'' and ''Gluconacetobacter'' also have a wide range of ethanol tolerances.  It is documented that ''Acetobacter'' and other acetic acid bacteria are able to grow in wine above 10% ABV, although is is not likely that acetic acid bacteria can grow in wines that are 15% ABV or above.  It is considered that no strains of acetic acid bacteria can grow in 15.5% ABV or above <ref>[https://www.sciencedirect.com/science/article/pii/B9780123750211100098 Molecular Wine Microbiology.  Chapter 9 - Acetic Acid Bacteria.  2011, Pages 227-255.  DOI: https://doi.org/10.1016/B978-0-12-375021-1.10009-8.]</ref>. 
 +
 
 +
Most strains are not tolerant of glucose once it is at ~25% of the solution <ref name="Gullo_2005">Characterization of acetic acid bacteria in “traditional balsamic vinegar”.  Maria Gullo, Cinzia Caggia, Luciana De Vero, Paolo Giudici.  2005.</ref>.
  
 
==Role in Belgian Beer==
 
==Role in Belgian Beer==
(To do)
+
Acetic acid bacteria have been found in [[Spontaneous_Fermentation|spontaneously fermented]] beers from Belgium (lambic) as well as America (American Coolship Ales) and [[Flemish_Red-Brown_Beer|Belgian Flanders Red Brown Beers]]. They are generally found at the air to liquid interface within barrels where they can access oxygen in the headspace. In general, brewers try to limit the impact of acetic acid bacteria in beer due to the higher levels of acetic acid that they can produce <ref name="Bossaert_2019" />.
===Sub Heading===
 
(To do)
 
  
 
==Role in American Sour Beers==
 
==Role in American Sour Beers==

Latest revision as of 18:15, 24 April 2021

Acetobacter is a genus of obligatory aerobic bacteria that is in the family Actobacteraceae. This family is more commonly known as "acetic acid bacteria" (AAB). Other genera of AAB include Gluconobacter, Gluconacetobacter, Acidomonas, Asaia, Kozakia, Swaminathania and Saccharibacter [1].

POTENTIAL REFERENCES:

http://www.thebrewingnetwork.com/the-sour-hour-episode-28/ cleaning Acetobacter out of barrels (~47 mins in)


https://www.facebook.com/groups/MilkTheFunk/permalink/1287080151320196/?comment_id=1287271667967711&reply_comment_id=1288151671213044&comment_tracking=%7B%22tn%22%3A%22R%22%7D

https://www.facebook.com/groups/MilkTheFunk/permalink/1356322957729248/

https://www.homebrewersassociation.org/how-to-brew/sour-microbes-yeast-and-bacteria-explained/

Leads on fermentation: http://www.mdpi.com/2311-5637/4/3/66

Flanders Red Ale

Metabolism

Acetic acid is produced by acetic acid bacteria through the oxidation of carbon sources into ethanol, followed by the oxidation of ethanol into acetaldehyde, and finally through the oxidation of acetaldehyde into acetic acid. When ethanol is depleted, acetic acid bacteria can also convert glycerol into cellulose, acetic acid, and carbon dioxide. Acetic acid bacteria have also been linked to elevated levels of acetoin, a pleasant buttery flavor, as well as ethyl acetate [2].

Alcohol and Sugar Tolerance

Tolerance to ethanol begins to decline in most Acetobacter species and strains starting at about 10% ABV. However, this is species and strain dependent. For example, some strains of A. aceti and A. pasteurianus (80% of strains tested) are unable to grow in 10% ABV. Other genera of acetic acid bacteria such as Gluconobacter and Gluconacetobacter also have a wide range of ethanol tolerances. It is documented that Acetobacter and other acetic acid bacteria are able to grow in wine above 10% ABV, although is is not likely that acetic acid bacteria can grow in wines that are 15% ABV or above. It is considered that no strains of acetic acid bacteria can grow in 15.5% ABV or above [3].

Most strains are not tolerant of glucose once it is at ~25% of the solution [1].

Role in Belgian Beer

Acetic acid bacteria have been found in spontaneously fermented beers from Belgium (lambic) as well as America (American Coolship Ales) and Belgian Flanders Red Brown Beers. They are generally found at the air to liquid interface within barrels where they can access oxygen in the headspace. In general, brewers try to limit the impact of acetic acid bacteria in beer due to the higher levels of acetic acid that they can produce [2].

Role in American Sour Beers

As a Contaminate

Role in Vinegar

(This can be brief, and might get deleted completely since this isn't a vinegar wiki. However most Acetobacter knowledge probably comes from vinegar.)

See Also

Additional Articles on MTF Wiki

External Resources

References