Difference between revisions of "Laboratory Techniques"

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===Making Agar Plates===
 
===Making Agar Plates===
 
<youtube>-EKb6AcaEBg</youtube>
 
<youtube>-EKb6AcaEBg</youtube>
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* Making WLD plates:
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<youtube>iqLuTNUZa2E</youtube>
  
 
===Yeast/Bacteria Isolation===
 
===Yeast/Bacteria Isolation===

Revision as of 18:14, 15 August 2017

This page will focus on home lab and small brewery lab techniques.

Equipment

(To do)

Growth Media

Propogators

Techniques

(Videos provided by Bryan of Sui Generis blog and Zach Taggart.)

Asceptic Technique

Making Agar Plates

  • Making WLD plates:

Yeast/Bacteria Isolation

See Wild Yeast Isolation.

Yeast Banking

Cell Counting

Gram Staining

Shipping Cultures

DeWayne Schaaf recommends the following procedures for shipping cultures with the USA states and territories [1]:

  1. Use 15ml centrifuge tubes from Cynmar LLC - Wine & Brew. They are great quality and have a very low rate of leakage, especially when combined with electrical tape.
  1. Place each liquid filled tube into its own snack sized baggie to minimize cross contamination if they do happen to leak.
  1. Each of these smaller bags will be placed into a quart Ziplock.
  1. My main shipping container are Uline Poly bubble mailers. I don't tend to use ice packs as I've found them unnecessary when shipping in favorable temps (use ice packs during the summer).
  1. USPS is my preferred shipping method. Using first class shipping, my packages typically take no more than 3 days to reach somewhere in the USA and cost right around $3, and that includes a tracking number. Shipping rates are roughly the same for any USA state or territory. The 48 continental states, Alaska, Hawaii, Puerto Rico, and Guam all cost roughly the same.

See also:

See Also

Additional Articles on MTF Wiki

External Resources

References