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Laboratory Techniques

9 bytes removed, 16:24, 5 September 2017
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'''UV/VIS Spectrophotometer'''
===Growth Media===
====''Lactobacillus/Pediococcus''====
See [[Lactobacillus#Selecting_for_on_Agar|Rogosa SL Agar]]
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====''Saccharomyces''====
A wide variety of media's can be used for ''Saccharomyces''. Bromocresol Green can also be added to these medias. Most ''Saccharomyces'' cannot metabolize this dye, causing the colonies to stain green. Chloramphenicol can also be added to eliminate bacterial growth. Although not all of these media's are specifically for your average brewers ''Saccharomyces'', most strains should have no issues growing.
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====''Brettanomyces''====
A few different medias can be used to isolate ''Brettanomyces'' but DBDM media is commonly used. WLD with additions of cycloheximide can also be used.
Reference on DBDM: https://www.facebook.com/groups/MilkTheFunk/permalink/1805210829507123/?comment_id=1805397166155156&comment_tracking=%7B%22tn%22%3A%22R%22%7D
====Misc/Other====
* [https://www.facebook.com/groups/MilkTheFunk/permalink/1697319680296239/ MTF tips on autoclaving wort extract based media in such a way that prevents precipitation and darkening.]
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