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===Semi-Anaerobic Containers for Incubating Plates===
Anerobic chambers can be costly. Here is a method that are two DIY methods. The first achieves under 1% O<sup>2</sup>. This setup , and both methods will also prevent mold growth.
# The plating setup: Use 60mm plates; bigger plates might not fit.
# The anaerobic tools: short wide-mouth jam jar, rubber-lined lid.
# Place 1 or 2 [https://www.amazon.com/Oxy-Sorb-100-Pack-Oxygen-Absorber-100cc/dp/B00A2ME6N8 Oxy-Sorb 100-Pack Oxygen Absorber, 100cc] into the jar.
# Seal the jar with electrical tape.
# Incubate!
Afterwards, seal the package of Oxy-Sorb with a vacuum sealer. Otherwise they will begin to absorb oxygen <ref>[https://www.facebook.com/groups/MilkTheFunk/permalink/1841431972551675/ Bryan of Sui Generis Blog and Caroline (Zach) Taggart on DIY semi-aerobic environment for plating. Milk The Funk Facebook group. 10/05/2017.]</ref>.
An alternative method using a vacuum sealer and a jar lid adapter:
# The plating setup. I use Use 60mm plates; bigger plates might not fit.
# The anaerobic tools: short wide-mouth jam jar, lid, vacuum sealer, ball jar attachment.
# After inoculating, I loosely tape the lids in place, just so they don't fall off when I'm placing them in the jar.
# Purge the container with CO2 for 5-10 seconds with lid just ajar.
# Place ball jar adapter on jar and vacuum seal container.
# Incubate! This is our Craigslist egg incubator. Works great!
<gallery>
File:Short jam jar.jpg|Short, wide, jam jar.
File:Anaerobic Jar2.jpg
File:Anaerobic Jar3.jpg