Laboratory Techniques
This page will focus on home lab and small brewery lab techniques.
Equipment
(To do)
Growth Media
Lactobacillus
See Rogosa SL Agar
Saccharomyces
Brettanomyces
A few different medias can be used to isolate Brettanomyces but DBDM media is commonly used. WLD with additions of cycloheximide can also be used.
DBDM Media Recipe
| Chemical | Usage Amount |
|---|---|
| Yeast nitrogenous base (YNB) | 6.5 grams |
| Ethanol | 6% v/v |
| Cycloheximide | 10 mg |
| p-coumaric acid | 100 mg |
| Bromocresol Green | 22 mg |
| Agar | 20 grams |
| Distilled Water | Fill to 1000 ML |
WLD Media Recipe
| Chemical | Usage Amount |
|---|---|
| Cycloheximide | 4 grams |
| Yeast Extract | 4 grams |
| Pancreatic Digest of Casein(Pepton) | 5 grams |
| Dextrose | 50 grams |
| Monopotassium Phosphate | .55 grams |
| Potassium Chloride | 425 mg |
| Calcium Chloride | 125 mg |
| Magnesium Sulfate | 125 mg |
| Ferric Chloride | 2.5 mg |
| Manganese Sulfate | 2.5 mg |
| Bromocresol Green | 22 mg |
| Agar | 20 grams |
| Distilled Water | Fill to 1000 ML |
Reference on DBDM: https://www.facebook.com/groups/MilkTheFunk/permalink/1805210829507123/?comment_id=1805397166155156&comment_tracking=%7B%22tn%22%3A%22R%22%7D
Misc/Other
Propagators
Techniques
(Videos provided by Bryan of Sui Generis blog and Zach Taggart.)
Aseptic Technique
Making Agar Plates
- Making WLD plates:
Yeast/Bacteria Isolation
- Mark Trent details his method for isolating Brettanomyces from Saccharomyces from dregs or other mixed cultures.
- See Wild Yeast Isolation.
- See isolating Lactobacillus.
Making Your Own Medias
Although media's can be bought pre-made, you can also make these media's yourself. Media's can be either sterilized via an autoclave/pressure cooker or using sterile filtering. Keep in mind it can be difficult however to sterile filter some items such as yeast extract, peptone and brewer's grade DME. Because of this some just autoclave parts of the media while sterile filtering the rest into the sterilized media.
It is crucial you use aspect technique with your media once it is sterilized to prevent any contamination. If you are storing extra media for later use make sure to remake it every month or 2 if unused.
Yeast Banking
Cell Counting
Gram Staining
Shipping Cultures
DeWayne Schaaf recommends the following procedures for shipping cultures with the USA states and territories [1]:
- Use 15ml centrifuge tubes from Cynmar LLC - Wine & Brew. They are great quality and have a very low rate of leakage, especially when combined with electrical tape.
- Place each liquid filled tube into its own snack sized baggie to minimize cross contamination if they do happen to leak.
- Each of these smaller bags will be placed into a quart Ziplock.
- My main shipping container are Uline Poly bubble mailers. I don't tend to use ice packs as I've found them unnecessary when shipping in favorable temps (use ice packs during the summer).
- USPS is my preferred shipping method. Using first class shipping, my packages typically take no more than 3 days to reach somewhere in the USA and cost right around $3, and that includes a tracking number. Shipping rates are roughly the same for any USA state or territory. The 48 continental states, Alaska, Hawaii, Puerto Rico, and Guam all cost roughly the same.
For 5 gallon pitches or cultures that may still be a bit active creating cO2 Soda Preform tubes work very well. These are the same type of tubes White Labs uses for their homebrew pitches. https://www.amazon.com/Soda-Bottle-Preforms-Caps-30/dp/B008MB1QNY/ref=sr_1_1?ie=UTF8&qid=1504524577&sr=8-1&keywords=soda+test+tubes
See also:
See Also
Additional Articles on MTF Wiki
- Microscope
- PH Meter
- Wild Yeast Isolation
- Titratable Acidity
- Saccharomyces
- Brettanomyces
- Pediococcus
- Lactobacillus