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===Impact of Fermentation Temperature===
In ''Saccharomyces'' species, higher fermentation temperature has been associated with faster fermentation, higher growth rates, and ester formation. [https://onlinelibrary.wiley.com/doi/full/10.1002/jib.565 Tyrawa et al. (2019) ] set out to explore the impact of fermentation temperature on 7 beer strains (including BSI Drei and several isolates from commercial sour/saison/lambic beers) and 2 wine strains of ''B. bruxellensis'' (Fermentis US-05 was used as a control). Each strain was fermented in autoclaved 100% 2-row malt wort at a starting gravity of 1.050 and fermented at 15°C (59°F) versus 22.5°C (72.5°F) for 28 days. The pitching rate was 1.2 x 10<sup>7</sup> cells/mL. Each strain was genetically tested to ensure their species was correctly identified and that they were genetically distinct from each other <ref name="Tyrawa_2019">[https://onlinelibrary.wiley.com/doi/full/10.1002/jib.565 The temperature dependent functionality of Brettanomyces bruxellensis strains in wort fermentations. Caroline Tyrawa, Richard Preiss, Meagan Armstrong, George van der Merwe. 2019. DOI: https://doi.org/10.1002/jib.565.]</ref>.
Their results showed that there is a vast diversity in how temperature effects attenuation for different strains of ''B. bruxellensis''. In general, the cooler 15°C (59°F) fermentation temperature slowed the attenuation rate for most strains. The US-05 attenuated the most at both temperatures, with only one saison strain matching that attenuation level when fermented at 22.5°C (72.5°F). This same strain, which was isolated from a commercial USA saison beer, and the BSI Drei strains had fast attenuation rates that were comparative to the US-05 fermentation at both temperatures, while the other strains had lag times of 8-10 days at 15°C (59°F) or 2-4 days at 22.5°C (72.5°F) <ref name="Tyrawa_2019" />.