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Lactobacillus

453 bytes added, 11:19, 22 September 2015
added clarification on Lacto starter media
===General Advice===
====Starters and Pitching Rate====
In addition to the starter information given in the [[Lactobacillus#Manufacturer_Tips|Manufacturer Tips]] above, this section includes general advice for ''Lactobacillus'' starters for homebrewers and brewers without access to MRS media. For growing ''Lactobacillus'' in a lab environment, or from an initial grouping of cells from a plate/slant, MRS media is much more expensive than the materials required most efficient growth media. However, for the method below (DMEfull pitches of ''Lactobacillus'' in beer/wort, apple juicebrewers probably don't want to add that much MRS media to their beer <ref>[https://www.facebook.com/groups/MilkTheFunk/permalink/1152135114814701/?comment_id=1152674674760745&offset=0&total_comments=9&comment_tracking=%7B%22tn%22%3A%22R0%22%7DConversation with Lance Shaner on Lacto starters on MTF. 09/22/2015.]</ref>. Therefore, chalk, and yeast nutrients)growing ''Lacto'' in a DME-based starter media is recommended. See [[Lactobacillus#External_Resources|External Resources]] for additional starter guides.
Pitching ~0.5-1 liter per ~20 liters of wort (~0.75-1 gallon per barrel) of ''Lactobacillus'' starter is the general guideline. The exact advisable pitching rates of commercial cultures may differ from manufacture to manufacturer. <ref>[https://www.facebook.com/groups/MilkTheFunk/permalink/1077639885597558/ MTF thread started by Brad Primozic. 5/29/2015.]</ref>. Counting the exact number of cells can be difficult to achieve due to the small size of bacteria cells, so starter volumes are generally used instead when talking about pitching rates for ''Lactobacillus'' <ref>[https://www.facebook.com/groups/MilkTheFunk/permalink/1068323126529234/?comment_id=1068337639861116&offset=0&total_comments=47&comment_tracking=%7B%22tn%22%3A%22R9%22%7D Conversation on MTF with Bryan Heit. 5/6/2015.]</ref>.

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