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* Not all strains can ferment maltose, which is almost 50% of the sugar composition of wort. These strains should be avoided for 100% ''Brettanomyes'' fermentations. See [[Brettanomyces#Carbohydrate_Metabolism|''Brettanomyces'' carbohydrate metabolism]] for more details.
* [http://brettanomycesproject.com/dissertation/pure-culture-fermentation/impact-of-pitching-rate/ Chad Yakobson's thesis] showed that WLP645, WLP650, WLP653, WY5112, WY5526, and WY5151 were not able to attenuate wort more than 50% within 35 days (these were pure cultures). BSI Drie was the only strain tested that was able to attenuate wort at levels similar to brewers yeast. All strains that he tested were able to utilize maltose, however some less efficiently than others. More time may or may not have resulted in further attenuation. Contamination with another yeast is one explanation for why brewers are able ot use these cultures from labs to fully attenuate wort (Yakobson used purified isolates for this research).
* [https://www.facebook.com/groups/MilkTheFunk/permalink/1935201276508077/ Nick Mader of Fremont Brewing (2017 Master Brewers Conference Presentation)] observed that 100% BSI Drei fermentation resulted in around 77% attenuation (3.17°P final gravity), while co-fermentation with different pitch rates of a saison yeast resulted in around ~90% attenuation (~1.5°P final gravity). The esters were generally lower than when cofermented with the saison yeast, but ethyl decanoate (apple, brandy) was considerably higher with the 100% fermentation with BSI Drei. 4-ethyl phenol concentrations with 100% Drei were around the same as when cofermented with the saison yeast. See also [[Brettanomyces_and_Saccharomyces_Co-fermentation#Review_of_Scientific_Analysis|cofermentation with ''Saccharomyces'']].