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Alternative Bacteria Sources

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==Yogurt Souring==
[[File:Green_man_berliner.jpg|thumb|Mike Karnowski from Green Man Brewery Blueberry Berliner Weisse made from a yogurt starter]]''Yogurt Souring'' refers to the method of souring wort using unpasteurized yogurt. Greek yogurt is often made with ''Lactobacillus acidophilus'', a lactic acid producing bacteria that is also associated with some health benefits <ref>[http://en.wikipedia.org/wiki/Lactobacillus_acidophilus Lactobacillus acidophilus Wikipedia page. Retrieved 3/3/2015.]</ref>. Using cultures of ''L. acidophilus'' from yogurt reportedly can make a 3.0-3.5 pH sour wort in 24 hours, without producing vomit/fecal flavors and aromas. To sour 5 gallons of wort with yogurt, make a 1 liter batch of unhopped starter wort the day before brew day. Add 2-4 teaspoons of live yogurt to the starter wort. Maintain a 100-110°F (37.8-43.3°C) temperature for about 24 hours. On brew day, and after the 24 hour sour starter is finished, mash and sparge a low IBU wort as normal, boil for a few minutes, and then chill the wort down to 100-110°F (37.8-43.3°C). Pitch the yogurt starter into the wort, and hold the temperature as close to the 100-110°F (37.8-43.3°C) range as possible. Bubbling CO2 through the wort is advised if possible to prevent potential off flavors, but is not required. Within 24 hours, the wort should be down in the 3.x pH range. Boil the wort, adding any hops that the recipe calls for, yeast nutrient, etc., and then cool the wort down to Saccharomyces pitching temperatures. Bob's your uncle! <ref>[https://www.facebook.com/groups/MilkTheFunk/permalink/1029598090401738/?comment_id=1030074730354074&offset=0&total_comments=25 Conversation with Mike Karnowski of Green Man Brewery on the MTF Facebook group. 3/3/2015.]</ref>
Many strains of ''L. acidophilus'', which is one of the more common species of ''Lactobacillus'' found in yogurt, produce toxins that can kill other species of ''Lactobacillus''. See [[Lactobacillus#Bacteriocins|''Lactobacillus'' bacteriocins]] for more information.
==Culturing ''Lactobacillus'' From Grains==
[[File:Sourgrains.jpg|thumb|100px|Derek Springer's grain starter]] If the brewer wants to use the LAB found naturally on the husks of grains, and doesn't want to risk pitching grains into the entire batch of wort, a Lacto starter culture with grain can be made. The benefit of this is that if the culture contains any vomit, fecal, or putrid aromas, it can be thrown away and tried again. If the starter needs to be dumped, try again with a different malt source; microbial populations can vary greatly between maltsters, harvest years, and malt type <ref>[[Grain#Microbial_Populations_on_Barley|Microbial Populations on Barley]]</ref>. The following is [http://www.fivebladesbrewing.com/lactobacillus-starter-guide/ Derek Springer's grain starter processon the Five Blades Brewing blog] <ref>[https://www.facebook.com/groups/MilkTheFunk/permalink/1050331444995069/?comment_id=1050399004988313&offset=0&total_comments=18&comment_tracking=%7B%22tn%22%3A%22R9%22%7D Conversation with Derek Springer on Milk The Funk. 4/4/2015.]</ref>:
# In a 2L flask make a standard starter wort (1.040 OG).
# Cap with an airlock.
# Keep as warm as you can for 2-3 days, ~110°F is best.
# After 2-3 days, strain the grains out using a colander. If no off aromas are detected, pitch the entire starter into the wort using one of the methods described on the [[Sour WortingWort Souring]] page.
If the starter is not going to be used right away, storing it in the fridge for up to a couple of weeks should be fine. If the starter still smells sweet/vegital then it should be ok to use. However if the starter starts to produce aromas of feces or vomit, do not use it <ref>[https://www.facebook.com/groups/MilkTheFunk/permalink/1474146382613571/?comment_id=1474383142589895&comment_tracking=%7B%22tn%22%3A%22R4%22%7D Conversation with Bryan of Sui Generis Blog on MTF. 11/18/2016.]</ref>.  :'''Safety note''': do not drink wort from grain starters. It has been reported that wort soured from grain often carries live ''Salmonella bongori'' for at least two days after souring, which is probably not always safe to consume any of the grain starter until associated with non-lethal food poisoning. This microbe will die during or after it has become beer (see primary fermentation See [[Grain#Malt_Inoculated_Wort|Malt Inoculated Wort]] and [[Wild_Yeast_Isolation#Safety|Wild Yeast Starters and Safety]])for more information.''
For science, see also:
# Incubate at a temperature of ~100°F for 24 hours (room temperature may work too).
# This results in a starter pH of about 3.2.
# Add the entire starter to wort using one of the methods described on the [[Sour WortingWort Souring]] page.
* See [https://www.facebook.com/groups/MilkTheFunk/permalink/1219357984759080 this MTF thread for more discussion/experiences on souring beer with kefir.]
# Incubate with an airlock at a temperature around ~90°F.
# Wait until the starter clears. This may take several days. When it clears, the starter is done. It may show signs of yeast fermentation.
# After fermentation finishes, add the entire starter to the wort. Reference the [[Sour WortingWort Souring]] page for further brewing procedures.
[https://www.facebook.com/groups/MilkTheFunk/permalink/1186384568056422/ Alex Loijos described an interesting process on MTF] of culturing successive microbes from a starting batch of sauerkraut. After preparing his sauerkraut, each day he took a sample of the developing brine and pitched it into the same batch of wort. This most likely had the effect of pitching ''Leuconostoc'' and ''Weissella'' species during days 1-3, and then ''Lactobacillus'' species starting on day 7.
See [http://suigenerisbrewing.blogspot.ca/2015/07/choosing-right-probiotics-for-souring.html this Sui Generis Blog article on which Probiotics to avoid based on the genera of microbes they contain]. Probiotics should have their contents listed plainly on their packaging. Avoid probiotics for animals as they tend to contain organisms that produce off-flavors such as ''Enterococcus'', ''Clostridium'', or ''Bacillus'' <ref>[https://www.facebook.com/groups/MilkTheFunk/permalink/1195902047104674/?comment_id=1195949307099948&comment_tracking=%7B%22tn%22%3A%22R%22%7D Conversation with DeWayne Schaaf on MTF. 12/17/2015.]</ref>. If [[Wort Souring|souring wort]], also avoid any probiotics that have ''Saccharomyces'' species in them because lactic acid bacteria can have a tough time quickly producing acidity if it is competeing with an active yeast fermentation.
===MTF "Reverse Kettle SourMTF Method"===Devin Bell has experienced getting good sourness by co-pitching probiotics with ''L. plantarum'' with yeast, or even after primary fermentation (also known as the "Reverse MTF Sour"). It is claimed that this method allows the yeast character Moved to be expressed more so than with kettle sours. In the case of pitching ''L. plantarum'' after fermentation, the beer turned out like a sour saison, where as co-pitched makes for a better Berliner Weisse or Gose style beer without the "saison" yeast character. This has also improved head retention in his beers. Using no hops seems to be required in order to get acid production from the ''L. plantarum'' probiotics after primary fermentation. Devin clarified that his "best success" is pitching ''S. cerevisiae'' saison strain with a selection of [[Mixed_Fermentation#Souring_Without_Brettanomyces|Mixed Fermentation; Souring Without ''Brettanomyces'' for primary fermentation. After 5-7 days of fermentation, he pitches ''L. plantarum'' (2 shots of GoodBelly or 1 package of Omega Labs OYL-605 for 5-6 gallons of beer) <ref>[https://www.facebook.com/groups/MilkTheFunk/permalink/1739156616112545/?comment_id=1739183316109875&reply_comment_id=1739749539386586&comment_tracking=%7B%22tn%22%3A%22R%22%7D Devin Bell. Milk The FunkFacebook group. 06/30/2017.]</ref>. Once terminal gravity is reached (1.002-1.004), he bottles right away. The bottles can be served at 8 weeks in the bottle, but start to peak at 24 weeks. This process is really more of a short term [[Mixed Fermentation]] <ref>[https://www.facebook.com/groups/MilkTheFunk/permalink/1562696320425243/?comment_id=1562702310424644&comment_tracking=%7B%22tn%22%3A%22R0%22%7D MTF discussion with Devin Bell on "reverse MTF kettle sour. 01/26/2017.]</ref>. See also [https://www.facebook.com/groups/MilkTheFunk/permalink/1217518078276404/ this thread by Devin Bell] and [https://www.facebook.com/groups/MilkTheFunk/permalink/1728489143845959/ this thread by Caroline Whalen Taggart]. * [https://www.facebook.com/SouthernBrewersConference/videos/881235365357984/ Devin Bell and Dan Pixley presentation at SBC 2017 on quick souring, including more details on this method.]
===General Tips and Experiences on Using Probiotics===
* For GoodBelly shots, only 1-2 shots (or 8 oz from a 32 oz carton) for 5 gallons of wort are needed (~20 billion cells for 5 gallons). That scales to a 1.5 - 2 cartons per BBL for the commercial scale (although some have reported that just over 1 carton per BBL is enough; freshness plays a factor) <ref>[https://www.facebook.com/groups/MilkTheFunk/permalink/1821240741237465/ Various MTF Members. Milk The Funk thread on how many cartons of GoodBelly to use. 09/14/2017.]</ref>. If using ''fresh'' GoodBelly that has been stored cold, don't bother making a starter (by checking to see if the pH gets lower, a starter will tell you if the bacteria is viable, but it will also increase the possibility of contamination). Mango is the preferred flavor because it imparts little flavor to the beer, but others can be used as well <ref>[https://www.facebook.com/groups/MilkTheFunk/permalink/1390440970984113/ Conversation on MTF about how many GoodBelly shots to use for kettle souring. 08/29/2016.]</ref>.
* Starters can be avoided for ''fresh'' tablet probiotics that have been stored cold, although measuring the pH of a starter will verify that the probiotic pills are still good to use. For Swansons L. plantarum tablets (5 billion cells per tablet), 7-10 tablets for 5 gallons of wort and held at 80-100°F for 1-2 days reportedly works well (temperature control and very warm temperatures are not necessary for ''L. plantarum'' in general) <ref>[https://www.facebook.com/groups/MilkTheFunk/permalink/1217518078276404/?comment_id=1217577824937096&reply_comment_id=1440146586013551&comment_tracking=%7B%22tn%22%3A%22R0%22%7D MTF thread on how many Swansons tablets can be pitched directly without a starter. 01/20/2016.]</ref>.
* Both liquid and pill format probiotics are extremely shelf unstable. They should be stored cold. Liquid probiotics may not be viable after two weeks of cold storage (see expiration date on the package). If liquid or pill format probiotics are stored at room temperature or warmer for any length of time, they may not be viable and a starter is recommended to see if they will acidify wort <ref>[https://www.facebook.com/groups/MilkTheFunk/permalink/1448945931800283/?comment_id=1448963338465209&comment_tracking=%7B%22tn%22%3A%22R22R4%22%7D Conversation with Devin Bell about storage and viability of probiotics. 10/25/2016.]</ref>. Note that [[Lactobacillus#Culture_Charts|cultures from brewing labs]] have reportedly better shelf lives than probiotics <ref>[https://www.facebook.com/groups/MilkTheFunk/permalink/1457254734302736/?comment_id=1458550380839838&comment_tracking=%7B%22tn%22%3A%22R%22%7D Conversation with Malcolm Frazer on MTF regarding shelf life of OYL Lacto. 11/03/2016.] </ref>.
* According to a representative of GoodBelly™ on MTF: "You're guaranteed 20 billion (cells) at time of consumption (often more)... We know that our products are within the claimed probiotic count range during shelf-life but every product has different count stability characteristics. The dynamics of the change/decline in cell counts is very different at room temperature than it is at refrigeration temperature... Fizziness may come from high metabolic activity or growth of Lp299v. A fizzy product may or may not have elevated counts as – at some point – the counts may go down again when high levels of lactic acid are in the product...StraightShot and our SuperShots are the most (cell count) stable. " <ref>[https://www.facebook.com/groups/MilkTheFunk/permalink/1745396375488569/?comment_id=1746279895400217&comment_tracking=%7B%22tn%22%3A%22R0%22%7D Marla and Armin from GoodBelly™. Milk The Funk Facebook group. 07/05/2017.]</ref>
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===Health Benefit Claims===
* [https://www.academia.edu/10204086/Lactic_Acid_Bacteria_in_Pharmaceutical_Formulations_Presence_and_Viability_of_Healthy_Microorganisms_?email_work_card=title Lactic Acid Bacteria in Pharmaceutical Formulations: Presence and Viability of "Healthy Microorganisms".]
* [http://suigenerisbrewing.com/index.php/2020/11/22/probiotic-beer/ "Probiotic Beer?" by immunologist Bryan Heit, Ph.D. on his blog "Sui Generis Blog".]
* [https://www.homebrewersassociation.org/zymurgy-magazine/jul-aug-2015/ "Is Beer a Probiotic?" by Matthew Farber, Ph.D. and Derek Dellinger; July/Aug issue of Zymurgy Magazine.]
==Using Sourdough Starter==
* [https://www.facebook.com/groups/MilkTheFunk/?post_id=3810254629002723 Kristoffer Krogerus talks about his lab's study that found sourdough cultures lack the ability to ferment maltose, which could be good for low alcohol beverages.]
* [http://brouwerij-chugach.com/?p=1001 Brian Hall's experience using a sourdough starter.]
* [https://www.facebook.com/groups/MilkTheFunk/permalink/1372578159437061/ MTF thread on using sourdough starters, with links to other threads.]
* [http://yourwildlife.org/2017/09/getting-the-measure-of-sourdough-biodiversity/ Article on the biodiversity of sourdough cultures.]
* [https://www.facebook.com/groups/MilkTheFunk/permalink/4139918236036359/ Evidence that sourdough yeast tend to be better at fermenting maltose than industrial/commercial bread yeasts.]
==Using Ginger Bug==
==Kombucha SCOBY==
Reports so far seem to indicate that fermenting with limited exposure The microbiome of kombucha SCOBYs varies from culture to oxygen produces better resultsculture. Since SCOBYThey can include various yeasts, including ''Saccharomyces'', ''Saccharomycodes'', ''Schizosaccharomyces'', ''Zygosaccharomyces'', ''Brettanomyces/Dekkera'', ''Candida'', ''Torulospora'', ''Kloeckera'', ''Pichia''s contain , ''AcetobacterMycotorula'', fermenting with exposure to too much oxygen produces a very vinegar tasting beverageand ''Mycoderma''. Another approach is to ferment with exposure to air until the beer tastes They also commonly contain some sort of acetic acid bacteria such as acidic as intended and then use an airlock to stop the aerobic fermentation; this will allow the ''Gluconobacter'', ''Gluconacetobacter'', or ''Acetobacter'' species, which form the "leather pancake"-like SCOBY of kombucha. Some kombuchas (30%) have also been found to be more active and have a greater affect on the flavor species of the beer since it needs oxygen in order to ferment ''Lactobacillus'' <ref>Private correspondence with Ron Davis by Dan Pixley[http://onlinelibrary.wiley.com/doi/10. 071111/061541-4337.12073/2017full A Review on Kombucha Tea—Microbiology, Composition, Fermentation, Beneficial Effects, Toxicity, and Tea Fungus. Rasu Jayabalan, Radomir V. Malbasa, Eva S. Loncar, Jasmina S. Vitas, Muthuswamy Sathishkumar. 2014.]</ref>. Another method for controlling the amount of acetic acid is blending finished kombucha with finished beer.
The microbiome of kombucha SCOBYs varies from culture Reports so far seem to indicate that fermenting with limited exposure to cultureoxygen produces better results. They can include various yeasts, including Since SCOBY's contain 'Saccharomyces'', ''SaccharomycodesAcetobacter'', ''Schizosaccharomyces'', ''Zygosaccharomyces'', ''Brettanomyces/Dekkera'', ''Candida'', ''Torulospora'', ''Kloeckera'', ''Pichia'', ''Mycotorula'', and ''Mycoderma''fermenting with exposure to too much oxygen produces a very vinegar tasting beverage. They also commonly contain some sort of acetic acid bacteria such Another approach is to ferment with exposure to air until the beer tastes as acidic as ''Gluconobacter'', ''Gluconacetobacter'', or intended and then use an airlock to stop the aerobic fermentation; this will allow the ''Acetobacter'' species, which form to be more active and have a greater effect on the "leather pancake"-like SCOBY flavor of kombucha. Some kombuchas (30%) have also been found the beer since it needs oxygen in order to have species of ''Lactobacillus'' ferment <ref>[http://onlinelibraryPrivate correspondence with Ron Davis by Dan Pixley.wiley.com/doi/10.1111 07/1541-4337.1207306/full A Review on Kombucha Tea—Microbiology, Composition, Fermentation, Beneficial Effects, Toxicity, and Tea Fungus. Rasu Jayabalan, Radomir V. Malbasa, Eva S. Loncar, Jasmina S. Vitas, Muthuswamy Sathishkumar. 20142017.]</ref>. Another method for controlling the amount of acetic acid is blending finished kombucha with finished beer.
* [https://www.facebook.com/groups/MilkTheFunk/search/?query=kombucha MTF Threads.]
* [http://fermentationnation.net/2015/06/episode-15-kombucha-lenny-boy-brewin Fermentation Nation podcast interview with Lenny Boy Brewing.]
* James Spencer on BasicBrewing Radio (kettle souring with a SCOBY):
:<youtubewidth="300" height="200">RUWMq91OhT8</youtube>
==Peppers==
==See Also==
===Additional Articles on MTF Wiki===
* [[Sour WortingWort Souring]]
* [[Commercial Sour Beer Dregs Inoculation]]
* [[Mixed Fermentation]]

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