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Brettanomyces

791 bytes added, 11:02, 2 April 2015
added a video on Mark Trent's homemade orbital shaker
It is worth noting that a "Malt Yeast Peptone Glucose" growth substrate has been shown to be a better substrate for growing an initial pitch of Brettanomyces. Specifically, when grown in wort, Brettanomyces will go through a 24 hour lag phase, a growth phase, another lag phase, and a second growth phase (all within 7-8 days). When grown in MYPG substrate, there is only a single growth phase and no lag phase, which has been reported by Yakobson to produce a larger cell count in the same amount of time <ref>[http://www.brettanomycesproject.com/2009/08/mypg-vs-wort-as-the-growth-substrate/ Yakobson, Chad. The Brettanomyces Project. MYPG Compared to Wort as a Growth Substrate. Retrieved 2/18/2015.]</ref>. Cells grown in MYPG also are better adapted to grow in wort <ref>[http://www.brettanomycesproject.com/dissertation/propagation-and-batch-culture-growth/propagation-discussion/ Yakobson, Chad. The Brettanomyces Project. Propagation and Batch Culture Discussion. Paragraph 5. Retrieved 2/18/2015.]</ref>. Practical instructions for making this substrate can be found on Jason Rodriguez's blog, "[http://sciencebrewer.com/2011/04/29/wild-yeast-project-mypg-culture-media/ Brew Science - Homebrew Blog]".
 
====Example of a Homemade Orbital Shaker====
Mark Trent's shaker platform (obtained from a used equipment outlet in Gilroy CA called "Outback Equipment" ) used to create a semi-aerobic environment for ''Brettanomyces''. Mark built an insulated box for it, and added temperature control. He can propagate up to 7 liters. This is running at 80 RPM as described in ''The Brettanomyces project'' <ref>[http://www.brettanomycesproject.com/dissertation/propagation-and-batch-culture-growth/propagation-methods/ Yakobson, Chad. The Brettanomyces Project. Propagation and Batch Culture Methods. Retrieved 2/18/2015.</ref><ref>[https://www.facebook.com/groups/MilkTheFunk/permalink/1048497155178498/ Discussion with Mark Trent on Milk The Funk. 4/2/2015.]</ref>.
 
<youtube>v3XoVYquNx4</youtube>
===Storing Brett===

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