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added image for Brett cell Counting with fluorescents
====Cell Counting====
[[File:Martyniak.JPG|thumb|300px|right|Bright-field and fluorescent images of ''B. clausenii'', ''B. bruxellensis'', and ''B. lambicus''. The fluorescent images show the counting of the yeasts with and without declustering the bud and pseudohyphae. Source: https://link.springer.com/article/10.1007/s10295-016-1861-4. Used with permission from Brian Martyniak.]]
The use of methylene blue, although popular in breweries, has been shown to be inaccurate when counting cells of ''Brettanomyces''. Trypan blue staining has been shown to give more accurate cell counting results <ref name="Hubbe" /><ref>[https://www.facebook.com/groups/MilkTheFunk/1407620505932826/?comment_id=1407723619255848&comment_tracking=%7B%22tn%22%3A%22R8%22%7D Discussion with Richard Preiss regarding counting Brettanomyces with methylene blue versus trypan blue staining. 09/15/2016.]</ref>.