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Brettanomyces secondary fermentation experiment

5 bytes removed, 12:14, 3 December 2015
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The beers will also be analyzed at a 3 month time point.
==Results/Discussion==
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File:Orgacids-omegaesc.png|'''Figure 5''' Measurement of volatile organic acids at 3 weeks.
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==Discussion==
At 3 weeks, conversion of 4-vinylguiacol (4-VG) to 4-ethylguiacol (4-EG) was observed in all samples dosed with ''Brettanomyces'' (Figure 1). This indicates that regardless of pitch rate, conversion of above-threshold amounts of 4-VG (clove) produced by ''Saccharomyces'' was metabolized to above-threshold levels of 4-EG (spicy, smoky). Additionally, ''de novo'' production of 4-ethylphenol (4-EP, barnyard/medicinal) was observed at around sensory threshold levels regardless of ''Brettanomyces'' pitch rate when compared to the ''Saccharomyces'' control. Taken together, these results indicate that volatile phenol production does not correlate to pitch rate in the range tested, as early as 3 weeks into secondary fermentation.
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