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Lactobacillus

41 bytes added, 15:48, 22 August 2017
added new subsection "Tolerance of Extreme Temperature'
Cell growth can also be influenced by the presence of other microorganisms, such as ''Saccharomyces'' and ''Brettanomyces''. One study by Hübbe showed that ''L. brevis'' and ''L. parabrevis'' grew to the normal high cell counts when grown individually and without competition. When co-fermented with ''Brettanomyces'', the cell count of ''L. brevis'' was halved, and the growth rate of ''L. parabrevis'' was greatly diminished to about 15-20% (the pitching rate of ''Brettanomyces'' was also tested, and seemed to not have an effect on the ''Lactobacillus'' growth). When co-fermented with both ''S. cerevisiae'' and ''Brettanomyces'', the ''Lactobacillus'' growth was greatly diminished to about 2-13% of what the normal cell growth was without competition. This appears to correspond with anecdotal reports from brewers that some ''Lactobacillus'' species/strains do not compete well with yeast, especially ''S. cerevisiae'' <ref name="Hubbe"></ref>.
 
Most ''Lactobacillus'' species have a thermal death rate of ~145°F (63°C). Freezing without glycerol will kill most cells, but it is possible for a very small number of cold-resistant mutant cells to survive <ref>[http://fermentationnation.net/2015/11/episode-26-quality-assurance-w-jessica-davis-of-the-bruery/ Fermentation nation Podcast interview with Jessica Davis, QA for The Bruery.]</ref> (~1:19:00 in).
====Effects of Oxygen====
Peyer et al. (2017) examined the effects of the sugar content of brewer's wort on a strain of ''Lactobacillus amylovorous''. They found that the higher gravity of the wort increased the growth of the ''Lactobacillus'', and therefore also the lactic acid production. This increase was linear until the extract reached 16%, at which time the growth increase began to slow down. The higher growth and more lactic acid production in higher gravity wort was probably due to increased nutrients as well as a higher buffer capacity. This growth increase plateaued in wort that had 18-20% sugar content, which was likely due to osmotic stress on the cells <ref name="Peyer_2017">[http://www.asbcnet.org/publications/journal/vol/2017/Pages/ASBCJ-2017-3861-01.aspx Sour Brewing: Impact of Lactobacillus amylovorus FST2.11 on Technological and Quality Attributes of Acid Beers. Lorenzo C. Peyer, Martin Zarnkow, Fritz Jacob, David P. Schutter, Elke K. Arendt. 2017.]</ref>.
 
====Tolerance of Extreme Temperature====
Most ''Lactobacillus'' species have a thermal death rate of ~145°F (63°C). Freezing without glycerol will kill most cells, but it is possible for a very small number of cold-resistant mutant cells to survive <ref>[http://fermentationnation.net/2015/11/episode-26-quality-assurance-w-jessica-davis-of-the-bruery/ Fermentation nation Podcast interview with Jessica Davis, QA for The Bruery.]</ref> (~1:19:00 in).
====Storage====

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