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Mixed Fermentation

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Reusing a sour yeast cake can often provide great results. Brewers have reported success re-pitching on very old yeast cakes (2+ years) without getting off flavors from yeast autolysis. After several months, ''[[Saccharomyces]]'' tends to die off due to the low pH in a sour beer. The bacteria and [[Brettanomyces]] tend to survive the lower pH, and their cell counts can be high in even an old yeast cake (interesting, ''Brettanomyces'' remains more viable over time if it was co-fermented with ''S. cerevisiae'' than if it was fermented by itself; i.e. [[100%25_Brettanomyces_Fermentation|100% ''Brettanomyces'' beers]] <ref name="Hubbe"></ref>). By pitching new wort on an old sour yeast cake, these microbes (particularly the [[Lactobacillus]]) have access to the simple sugars in the wort <ref>[http://www.themadfermentationist.com/2009/11/brewing-sour-beer-at-home.html Tonsmeire, Michael. The Mad Fermentationist. Brewing Sour Beer at Home. Last paragraph in the "Inoculation" section. Retrieved 2/19/2015.]</ref>. Using a young yeast cake is also a viable option, and may carry over more surviving ''Saccharomyces'' cells as well as more viable cells of the other various microbes. In general, washing the yeast cake is not necessary. Beer itself can also be used as an inoculate, and might be more desirable so as to avoid trub. If the beer has sat in a barrel ''Acetobacter'' and other unwanted microbes might be more present on the surface of the pellicle, and would remain after racking the beer out of the barrel, so some professional brewers advise using beer as an inoculate for this reason <ref>[https://youtu.be/IGzoh4brILA?t=52m30s Yakobson, Chad. Interview on Craft Commander. 12/20/2016. Retrieved 12/22/2016.] (~52 mins in)</ref>.
Some brewers will harvest a certain amount of trub from their fermenters (500mL for example), and use only this amount to inoculate a new batch of beer. This will allow the brewer to control the amount of dead trub material that goes into the new beer. Michael Tonsmeire often advises that the brewer also pitch a fresh culture of ''Saccharomyces '' <ref>[http://www.themadfermentationist.com/2009/11/brewing-sour-beer-at-home.html Tonsmeire, Michael. The Mad Fermentationist. Brewing Sour Beer at Home. Comments section. Retrieved 2/19/2015.]</ref>.
Some brewers have good success reusing a yeast cake or a portion of a yeast cake by leaving the wort in contact with the old yeast cake for 1-4 days. After 1-4 days, a fresh culture of ''Saccharomyces'' is pitched to finish the fermentation. The 1-4 day head start gives the souring bacteria a head start and results in a low pH beer. The harvested yeast cake can also be pitched at the same time as a fresh culture of Saccharomyces, or afterwards. The decided timing on when to pitch the harvest sour yeast cake will effect the acidity of the sour beer: early pitching of the sour yeast cake generally produces a more acidic beer, and later pitching generally produces a less acidic beer.

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