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Pellicle

543 bytes added, 23:31, 24 September 2015
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An often asked question by homebrewers is can a contaminating microbe be identified based on the appearance of a pellicle. This is a difficult exercise for a few reasons.
# While different strains of the same species of microbe (at least in ''Acetobacter'') can create different types of polysaccharides that make up the pellicle, their overall chemical make up is more or less very similar.
# Numbers and size of bubbles in a pellicle is created by trapped CO2, and thus is not an identifying feature.
# Scientific research on pellicle formation in beer is currently next to none.
# Identification of microbe species and sometimes even genus under a microscope based on cell morphology alone is not enough to be certain of that identification (see [http://suigenerisbrewing.blogspot.com/2014/12/brett-trois-riddle-wrapped-in-mystery.html this article on Trois genera identification] as an example), let alone attempting to identify microbes based on a visual "macro-level" formation such as a pellicle.
# DNA analysis of a microbe is the only way to reliably identify the species of a microbe. This is why [http://bootlegbiology.com/2015/09/17/local-yeast-project-study-barrel-culture-t-shirts/ Bootleg Biology is launching a program to identify wild caught microbes through DNA analysis].
 
The above stated, it might be possible to make an educated guess as to the genus of microbe that produced a pellicle if there was a controlled study (same wort, same incubation conditions, etc.) that examined the visual differences between pellicles formed by different genre of microbes. Unfortunately, such a study has not been performed that we know of. Thus, it is unknown if the genus of a contaminating microbe can be determined based on the visual appearance of the pellicle alone <ref>[https://www.facebook.com/groups/MilkTheFunk/permalink/1153845284643684/?comment_id=1153891934639019&reply_comment_id=1154095574618655&total_comments=5&comment_tracking=%7B%22tn%22%3A%22R2%22%7D Conversation with Richard Preiss and others on MTF. 09/24/2015.]</ref>.
==Handling/Racking==

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