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Saccharomyces

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====''Saccharomyces cerevisiae'' var. ''diastaticus''====
''Saccharomyces cerevisiae'' var. ''diastaticus'' is a variant of ''S. cerevisiae'' that can ferment certain types of starches and dextrins, and has been identified as a contaminant in breweries and is responsible for a few large recalls. This variant can produce extracellular glucoamylase (also called [https://en.wikipedia.org/wiki/Alpha-glucosidase alpha-glucosidase], and is the same enzyme that ''[[Brettanomyces]]'' produces to break down starches and dextrins) that can break down the α-1,4 linkages of starches and dextrins releasing glucose that is then fermented by the yeast. When beer containing this yeast is packaged too early, it will continue to slowly ferment dextrins and cause over-carbonation. When pitching a proper cell count or pitching rate of a diastaticus yeast into wort, it will fully ferment as quickly or nearly as quickly as any other brewers yeast. The problem of slow fermentation in already packaged beer is only a concern when diastaticus is introduced as a very small cell count, for example as an accidental contamination <ref>[https://www.facebook.com/groups/MilkTheFunk/permalink/1982499288444942/?comment_id=1983013578393513&comment_tracking=%7B%22tn%22%3A%22R1%22%7D Caroline Whalen Taggart. Milk The Facebook post on how quickly diastaticus ferments. 02/09/2018.]</ref>. This enzyme is heat stable and can continue to work on starches and dextrins after pasteurization <ref>[https://www.mbaa.com/publications/tq/tqPastIssues/1983/Abstracts/tq83ab19.htm Factors That Control the Utilization Of Wort Carbohydrates by Yeast. G. G. Stewart, I. Russell, and A. M. Sills. MBAA Technical Quarterly, Vol. 20, No. 1, 1983.] </ref>. The capability to produce this enzyme is encoded by the STA1, STA2, or STA3 genes. Not all strains containing one of these genes produces the glucoamylase enzyme <ref>[https://link.springer.com/article/10.1007%2FBF00365634 STA10: A gene involved in the control of starch utilization by Saccharomyces. Julio Polaina, Melanie Y. Wiggs. 1983.]]</ref><ref>[http://onlinelibrary.wiley.com/doi/10.1002/yea.1102/full Structural analysis of glucoamylase encoded by the STA1 gene of Saccharomyces cerevisiae (var. diastaticus). Ana Cristina Adam, Lorena Latorre-Garcia, Julio Polaina. 2004.]</ref>. However, it has been reported by some microbiologists that most (if not all) brewing strains that contain STA1, STA2, or STA3 do produce the glucoamylase enzyme <ref>[https://www.facebook.com/groups/MilkTheFunk/permalink/1935201836508021/?comment_id=1936604203034451&reply_comment_id=1937166892978182&comment_tracking=%7B%22tn%22%3A%22R7%22%7D Richard Preiss. Milk the Funk thread about STA1 gene correlation to glucoamylase production. 12/31/2017.]</ref><ref name="mbaa_diastaticus">[http://masterbrewerspodcast.com/068-diastaticus-part-1 Matthew Peetz of Inland Island and Tobias Fischborn of Lallemand. "Master Brewers Association Podcast" 12/25/2017.]</ref>(~16 mins). This report is directly contradicted by White Labs regarding their strains with this gene [https://whitelabs.com/yeast-bank/wlp590-french-saison-ale-yeast WLP590], [https://www.whitelabs.com/yeast-bank/wlp099-super-high-gravity-ale-yeast WLP099], and [https://www.whitelabs.com/yeast-bank/wlp045-scotch-whisky-yeast WLP045] (it is likely that the strains reported by other microbiologists to be ''diastaticus'' are the same as these White Labs strains, but this has not been verified). There has been a report by Richard Preiss of [[Escarpment Laboratories]] that WLP570 and WLP585 both have the STA1 gene, but it takes weeks before they hyper-attenuate <ref>[https://www.facebook.com/groups/MilkTheFunk/permalink/1888017211226484/?comment_id=1911782002183338&reply_comment_id=1982274298467441&comment_tracking=%7B%22tn%22%3A%22R9%22%7D Richard Preiss. Milk The Funk Facebook group post on WLP570 and WLP585 being diastaticus. 02/08/2018.]</ref>. This yeast has also reportedly been detected using [http://onlinelibrary.wiley.com/doi/10.1002/j.2050-0416.1981.tb04005.x/pdf LCSM agar plates], although other species of wild ''Saccharomyces'' yeast can grow on this media <ref name="mbaa_diastaticus" />(~18 mins) and PCR DNA analysis is required to give a positive identification of ''diastaticus''. Documented attenuation percentages above 75% for any strain of ''S. cerevisiae'' is also an indicator that the strain is ''diastaticus'' <ref name="mbaa_diastaticus" />. Var. ''diastaticus'' contamination in breweries has been a recent hot topic, and the source of some contaminations has been suspected to come from certain yeast suppliers (namely White Labs) <ref>[https://www.courthousenews.com/wp-content/uploads/2017/11/Left-Hand-v-White-Labs-COMPLAINT.pdf District Court, Boulder County, Colorado. Case Number 2017CV31132. Filed 11/14/2017.]</ref>.
''S. cerevisiae'' var ''diastaticus'' can grow at 37°C and can also remain viable at refrigeration temperatures <ref>[http://www.ebc2017.com/inhalt/uploads/P095_Begrow.pdf Wade Begrow. "Recent notable microbiological contaminations of craft beer in the United States". Presentation poster at EBC 2017. Retrieved 11/19/2017.]</ref>. WY3711 saison yeast, which originated from Basserie Thiriez, has been determined to be this subspecies <ref name="preiss_diastaticus" />. In the [[Saccharomyces#Commercial_Farmhouse.2FBelgian_Strains_of_Saccharomyces|commercial culture]] lists below where we believe a lab is selling this strain of yeast or might be selling this strain of yeast, we note that it is determined/suspected to be ''diastaticus''. The Belle Saison strain from Lallemand is killer neutral, which means that killer wine strains will not kill it (it is not verified if Belle Saison is the same as WY3711/Thiriez, although it is suspected to be).
Some hints as to which White Labs products might be ''diastaticus'' have been deduced from the Gallone et al. study that published DNA sequencing on most of the yeast strains in the White Labs bank. However, the codes used in the Gallone paper for each strain of yeast were not defined as far as which White Labs products correspond to which code used in the study. Some of the codes have been speculated on which White Labs strains they might refer to (see [[Saccharomyces#History_of_Domestication|History of Domestication]] above). The strains from the Gallone paper that appear to contain STA1 are Beer002, Wine019, Beer092 and Beer059. The Beer059 code might correspond to WLP026 according to this speculative [https://www.facebook.com/groups/MilkTheFunk/permalink/1400297539998456/?comment_id=1908170505877821&comment_tracking=%7B%22tn%22%3A%22R0%22%7D table], which has had reports of high attenuation. It is not clear at this time whether this strain is ''diastaticus'' or not <ref>[https://www.facebook.com/groups/MilkTheFunk/permalink/1400297539998456/?comment_id=1909596632401875&reply_comment_id=1910328215662050&comment_tracking=%7B%22tn%22%3A%22R9%22%7D MTF thread on STA1 gene and White Labs strains that might have it or not. Milk The Funk Facebook group. 12/07/2017.]</ref>.
 
When pitching a proper cell count or pitching rate of a diastaticus yeast into wort, it will fully ferment as quickly or nearly as quickly as any other brewers yeast. The problem of slow fermentation in already packaged beer is only a concern when diastaticus is introduced as a very small cell count, for example as an accidental contamination <ref>[https://www.facebook.com/groups/MilkTheFunk/permalink/1982499288444942/?comment_id=1983013578393513&comment_tracking=%7B%22tn%22%3A%22R1%22%7D Caroline Whalen Taggart. Milk The Facebook post on how quickly diastaticus ferments. 02/09/2018.]</ref>.
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