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In 2007, the Australian team of Grbin et al. developed a complex and unique method of analyzing forms of THP using a Finnigan TSQ 70 mass spectrometer directly coupled to a Varian 3400 gas chromatograph. The chromatograph was equipped with a 30 m J&W Carbowax 20 CAM fused silica column, 0.25 mm i.d, and 0.25
μm film thickness. They were able to confirm that higher lysine levels increased the amount of ATHP produced (but not ETHP), although with diminishing increases of THP as the lysine level was increased. They also discovered that L-ornithine also functioned as a precursor for THP production in ''Brettanomyces''. The group proposed a biochemical pathway for the different forms of THP in ''Brettanomyces'' <ref name="Grbin_2007" />.
==Forms of THP==