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| Inoculation timing || After ''Saccharomyces'' has finished fermentation || At the start of Fermentation || The timing of the ''Brettanomyces'' pitch may not have a significant effect a lot of the time; pending data from George Van Der Merwe or another source of scientific data <ref>[https://www.facebook.com/groups/MilkTheFunk/permalink/2103639509664252/?comment_id=2117621214932748&comment_tracking=%7B%22tn%22%3A%22R%22%7D Richard Preiss. Milk The Funk Facebook thread on co-pitching versus subsequent pitching of ''Brettanomyces''. 06/04/2018.]</ref>.
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| ''Brettanomyces'' Inoculation cell count || Lower cell count or higher cell count || Higher cell count or lower cell count|| Although more data is needed, pitching rate of ''Brettanomyces'' may not have a measured impact on beer flavor. See [[Brettanomyces secondary fermentation experiment]].
See also:
* [https://www.youtube.com/watch?v=IudVmYyWXss Presentation from Caroline Tyrawa via Escarpment Laboratories Crowdcast video.]
===''Brettanomyces'' Strain Selection===
[https://ir.library.oregonstate.edu/downloads/gh93h631p Riley Humbert's Bachelors thesis] reported that the ability to hyper attenuate beer in secondary fermentation of ''B. bruxellensis'' is strain dependent with some strains fermenting at a slower rate than others (this was also the case for these strains when primary fermenting wort). The fermentation rate did not correlate to phenol production; for example, one strain that produced the most 4-vinyl phenol and 4 ethyl phenol was one of the slowest fermenters. Humbert also reported that strains of ''B. bruxellensis'' isolated from beer performed better than those isolated from wine, which is in agreement with previous experiments <ref>[https://ir.library.oregonstate.edu/downloads/gh93h631p Riley Humbert for the degree of Honors Baccalaureate of Science in Chemical Engineering presented on May 21, 2021. Title: Performance of Brettanomyces Yeast Strains in Primary and Secondary Beer Fermentations.]</ref>.
==See Also==