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Laboratory Techniques

576 bytes added, 00:29, 4 November 2022
Brettanomyces
See also: [[Wild_Yeast_Isolation#Wild_Brettanomyces|Wild Isolation of ''Brettanomyces'']].
 
'''YPD'''
 
See [https://suigenerisbrewing.com/index.php/2022/11/02/this-is-the-wild-brettanomyces-your-are-looking-for/ this article] by Dr. Bryan Heit on using a modified YPD media that is cheaper and easier to prepare than WLN, along with techniques to help improve the chances of culturing wild ''Brettanomyces'' while mostly eliminating other yeasts and mold.
 
{| class="wikitable"
|-
! Chemical !! Usage Amount
|-
| Yeast Extract || 10 grams
|-
| Peptone || 20 grams
|-
| Dextrose || 20 grams
|-
| Agar(optional) || 15 grams
|-
| Distilled Water || Fill to 1000 ML
|}
'''EBB Media Recipe'''

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