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Laboratory Techniques

380 bytes added, 07:16, 1 September 2017
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====''Saccharomyces''====
====''Brettanomyces''====
 
A few different medias can be used to isolate ''Brettanomyces'' but DBDM media is commonly used.
 
{| class="wikitable"
|-
! Chemical !! Usage Amount
|-
| Yeast nitrogenous base (YNB) || 6.5 grams
|-
| Ethanol || 6% v/v
|-
| Cycloheximide || 10 mg
|-
| p-coumaric acid || 100 mg
|-
| Bromocresol Green || 22 mg
|-
| Agar || 20 grams
|-
| Distilled Water || Fill to 1000 ML
|}
 
Reference on DBDM: https://www.facebook.com/groups/MilkTheFunk/permalink/1805210829507123/?comment_id=1805397166155156&comment_tracking=%7B%22tn%22%3A%22R%22%7D
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