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Laboratory Techniques

882 bytes added, 16:09, 5 September 2017
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===Growth Media===
 
====''Lactobacillus''====
See [[Lactobacillus#Selecting_for_on_Agar|Rogosa SL Agar]]
====''Saccharomyces''====
A wide variety of media's can be used for ''Saccharomyces''. Bromocresol Green can also be added to these medias. Most ''Saccharomyces'' cannot metabolize this dye, causing the colonies to stain green. Chloramphenicol can also be added to eliminate bacterial growth. Although not all of these media's are specifically for your average brewers ''Saccharomyces'', most strains should have no issues growing.
 
'''YPD Media'''
{| class="wikitable"
|-
! Chemical !! Usage Amount
|-
| Yeast Extract || 10 grams
|-
| Peptone || 20 grams
|-
| Dextrose || 20 grams
|-
| Agar(optional) || 15 grams
|-
| Distilled Water || Fill to 1000 ML
|}
 
'''MYPG Media'''
{| class="wikitable"
|-
! Chemical !! Usage Amount
|-
| Malt Extract || 3 grams
|-
| Yeast Extract || 3 grans
|-
| Peptone || 3 grams
|-
| Dextrose || 10 grams
|-
| Agar || 15 grams
|-
| Distilled Water || Fill to 1000 ML
|}
 
 
====''Brettanomyces''====
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