13,592
edits
Changes
added new section "Semi-Anaerobic Containers for Incubating Plates"
===Gram Staining===
<youtube>xgJyGCC-mbg</youtube>
===Semi-Anaerobic Containers for Incubating Plates===
[[File:Short jam jar.jpg|thumb|Short, jam jar used as an semi-anaerobic chamber. Photo provided by [https://www.facebook.com/groups/MilkTheFunk/permalink/1841431972551675/ Caroline (Zach) Taggart].]]
Anerobic chambers can be costly. Here is a method that achieves under 1% O<sup>2</sup>. This setup will also prevent mold growth.
# The plating setup: Use 60mm plates; bigger plates might not fit.
# The anaerobic tools: short wide-mouth jam jar, rubber-lined lid.
# After inoculating, loosely tape the lids in place, just so they don't fall off when placing them in the jar.
# Purge the jar with CO2 for 5-10 seconds with lid just ajar.
# Place 1 or 2 [https://www.amazon.com/Oxy-Sorb-100-Pack-Oxygen-Absorber-100cc/dp/B00A2ME6N8 Oxy-Sorb 100-Pack Oxygen Absorber, 100cc]] into the jar.
# Seal the jar with electrical tape.
Afterwards, seal the package of Oxy-Sorb with a vacuum sealer. Otherwise they will begin to absorb oxygen <ref>[https://www.facebook.com/groups/MilkTheFunk/permalink/1841431972551675/ Bryan of Sui Generis Blog and Caroline (Zach) Taggart on DIY semi-aerobic environment for plating. Milk The Funk Facebook group. 10/05/2017.]</ref>.
==Shipping Cultures==