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Laboratory Techniques

691 bytes added, 20:38, 5 October 2017
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===Semi-Anaerobic Containers for Incubating Plates===
[[File:Short jam jar.jpg|thumb|Short, jam jar used as an semi-anaerobic chamber. Photo provided by [https://www.facebook.com/groups/MilkTheFunk/permalink/1841431972551675/ Caroline (Zach) Taggart].]]
Anerobic chambers can be costly. Here is a method that achieves under 1% O<sup>2</sup>. This setup will also prevent mold growth.
# The plating setup: Use 60mm plates; bigger plates might not fit.
Afterwards, seal the package of Oxy-Sorb with a vacuum sealer. Otherwise they will begin to absorb oxygen <ref>[https://www.facebook.com/groups/MilkTheFunk/permalink/1841431972551675/ Bryan of Sui Generis Blog and Caroline (Zach) Taggart on DIY semi-aerobic environment for plating. Milk The Funk Facebook group. 10/05/2017.]</ref>.
 
An alternative method using a vacuum sealer and a jar lid adapter:
# The plating setup. I use 60mm plates; bigger plates might not fit.
# The anaerobic tools: short wide-mouth jam jar, lid, vacuum sealer, ball jar attachment.
# After inoculating, I loosely tape the lids in place, just so they don't fall off when I'm placing them in the jar.
# Purge the container with CO2 for 5-10 seconds with lid just ajar.
# Place ball jar adapter on jar and vacuum seal container.
# Incubate! This is our Craigslist egg incubator. Works great!
 
<gallery>
File:Short jam jar.jpg|Short, wide, jam jar.
File:Anaerobic Jar2.jpg
File:Anaerobic Jar3.jpg
</gallery>
 
''These methods were shared by Zach Taggart of 42 North Brewing Co. and [http://suigenerisbrewing.com/ Bryan of Sui Generis Blog]. See [https://www.facebook.com/groups/MilkTheFunk/permalink/1841431972551675/ this MTF thread] for details.''
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