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Laboratory Techniques

734 bytes added, 13:16, 7 April 2018
added Malt Agar Media Recipe to Brettanomyces media
===''Brettanomyces''===
A few different medias media can be used to isolate ''Brettanomyces'' but DBDM media medium is commonly used. WLD with additions of cycloheximide can also be used. A simple Malt agar with cycloheximide has been shown to grow ''Brettanomyces'' that has been adapted to the brewing environment more efficiently than Dekkera medium (recommended by the European Brewing Convention, but not listed) and universal beer medium (recommended by the Brewery Convention of Japan; also not listed) <ref>[https://www.tandfonline.com/doi/abs/10.1094/ASBCJ-2008-0917-01 Effects of Beer Adaptation on Culturability of Beer-Spoilage Dekkera/Brettanomyces Yeasts. Koji Suzuki, Shizuka Asano, Kazumaru Iijima, Tomoo Ogata, Yasushi Kitagawa & Tsunehiro Ikeda. 2018.]</ref>.
'''DBDM Media Recipe'''
|-
| Distilled Water || Fill to 1000 ML
|}
 
'''Malt Agar Media Recipe'''
{| class="wikitable"
|-
| Malt extract || 30 grams
|-
| Cycloheximide || 0.01 grams
|-
| Agar || 15 grams
|-
|}

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