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Laboratory Techniques

10 bytes added, 13:28, 7 April 2018
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===''Brettanomyces''===
A few different media can be used to isolate ''Brettanomyces'' but DBDM medium is commonly used. WLD with additions of cycloheximide can also be used. A simple Malt agar with cycloheximide has been shown to grow ''Brettanomyces'' that has been adapted to the brewing environment more efficiently than Dekkera medium (recommended by the European Brewing Convention, but not listedhere) and universal beer medium (recommended by the Brewery Convention of Japan; also not listedhere) <ref>[https://www.tandfonline.com/doi/abs/10.1094/ASBCJ-2008-0917-01 Effects of Beer Adaptation on Culturability of Beer-Spoilage Dekkera/Brettanomyces Yeasts. Koji Suzuki, Shizuka Asano, Kazumaru Iijima, Tomoo Ogata, Yasushi Kitagawa & Tsunehiro Ikeda. 2018.]</ref>.
'''DBDM Media Recipe'''

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