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Landrace Yeast

2,549 bytes removed, 15:15, 12 September 2019
updated Muri
===Bjarne Muri's Olden Farmhouse Yeast===
It was initially found by Krogerus et al. (2018) that the single Muri strain recovered by Bjarne Muri is a hybrid of ''S. cerevisiae'' and ''S. uvarum'', and is most closely related to the beer 2 yeast group (kveik falls into the Beer 1 group; see [http[Saccharomyces#History_of_Domestication|History of Domestication]] for more information on the beer yeast genetic groups) <ref>[https://www.garsholfrontiersin.org/articles/10.3389/fmicb.2018.02253/full A Unique ''Saccharomyces cerevisiae'' × ''Saccharomyces uvarum'' Hybrid Isolated From Norwegian Farmhouse Beer: Characterization and Reconstruction.priv Kristoffer Krogerus, Richard Preiss, Brian Gibson. 2018. DOI: https://doi.noorg/blog10.3389/406fmicb.html Full write up 2018.02253.]</ref>. Unlike kveik, Muri is POF+ (phenolic) and STA1 (diastaticus). Krogerus then performed full genome sequencing on the Muri strain and discovered that it is a genetic match for WLP351 Bavarian Weizen yeast. This strain is either a contaminant or the original yeast used by Lars Garsholthe Muri family was the same as the WLP351 Bavarian Weizen strain.]
"Muri is an interspecies hybrid between ''SSee [https://beer. cerevisiae'' and ''Ssuregork. uvarum'com/?p=4094 Kristoffer Krogerus'. Hybrids of this combination have previously been isolated from wine fermentationss blog post] that goes into detail on his findings, but there are limited reports of any from brewing environments. ''Sas well as his [https://www. uvarum'' is closely related to ''Sfacebook. eubayanus'', one of the parents of lager yeast (which is also a hybrid)com/groups/MilkTheFunk/permalink/2906356522725876/ Q&A post on MTF].
Phylogenetic analysis of the two parental genomes of Muri revealed that the ''S. cerevisiae'' parent appears to belong to the Beer2 group, and is most closely related to various English ale strains. The parent strain was therefore not related to any of the other (sequenced) kveik isolates. It is not included in the paper, but Muri is also different to e.g. the S6U ''S. cerevisiae'' × ''S. uvarum'' hybrid that was isolated from wine, where the ''S. cerevisiae'' parent groups in the Wine clade. The ''S. uvarum'' parent was most closely related to Central European ''S. uvarum'' strains used for cider making (unfortunately ''S. uvarum'' strains haven't been sequenced as much as ''S. cerevisiae'' strains).Background information:  The ''S* [http://www. uvarum'' genome of Muri also contains a lot of introgressions from ''Sgarshol. eubayanus''priv. This is common in domesticated ''Sno/blog/406. uvarum'' strains. Muri, for example, appears to have a chimeric chromosome 7, where half is from ''S. uvarum'' and half from ''S. eubayanus''. We then obtained some of the closely related ''S. cerevisiae'' and ''S. uvarum'' strains, as well as some other hybrids, and compared their phenotypic properties (with focus html Original write up on those related to brewing). Muri is POF+, diastatic (STA+), has a wide temperature range of growth, good flocculation and good ethanol tolerance. The ''S. cerevisiae'' and ''S. uvarum'' strains that were genetically most similar to Muri also were the most similar phenotypically. These are named A241 and C995 in the paper, respectively. We then attempted to recreate the Muri hybrid by creating new hybrids between A241 and C995. We then compared Lars Garshol before the newly created hybrids with Muri, and found genetic discovery that the new hybrids were more similar to Muri than either of the parent strains. However, they still differed in some respects (e.g. low temperature wort fermentations). There are several potential reasons for this, e.g. the heterozygosity of the parent strains, the sequence divergence between Muri and the parent strains, the effect of the ''S. eubayanus'' introgressions, and the mitochondrial inheritance. This approach of creating new hybrids that mimic an existing one might not be very valuable for the average brewer, but it is something that could be useful in researching how yeast hybrids adapt and change. For example, one could try to recontruct lager yeast in a similar fashion, and see what kind of conditions give rise to similar genetic changes that have occured in the lager yeast during the hundreds of years the hybrids have existedsame as WLP351 Bavarian Weizen.] So to sum up, Muri is a hybrid and is not related to other kveik strains (there is the possibility of contamination when Bjarne tried to revive his kveik culture, but the strain is nevertheless interesting and unique). The ''S. cerevisiae'' x ''S. uvarum'' combination is rare in brewing. Muri is POF+ and diastatic, so might work well for saisons or other beer styles where 4VG and a dry finish is desired." ~ Kristoffer Krogerus summary of his published study <ref>* [https://www.facebook.com/groups/MilkTheFunk/permalink/2292973420730859/ MTF post by Kristoffer Krogerus. Milk The Funk Facebook group post on the whole genome sequencing results for that explains their 2018 study that discovered that Muri. 09/24/2018.]</ref>is a hybrid yeast that is not genetically related to other kveik strains, [https://wwwincluding process and discoveries.frontiersin.org/articles/10.3389/fmicb.2018.02253/full A Unique ''Saccharomyces cerevisiae'' × ''Saccharomyces uvarum'' Hybrid Isolated From Norwegian Farmhouse Beer: Characterization and Reconstruction].
Yeast banking and commercial availability:
* [https://catalogue.ncyc.co.uk/saccharomyces-cerevisiae-4045 NCYC 4045 - One strain of ''Saccharomyces cerevisiae''.]
** Also It is packaged by [https://www.whitelabs.com/ White Labs] as WLP6788 and available from [http://www.bryggselv.no/ Bryggselv.no]. US customers can send an email to "post @ bryggselv.no". See [http://www.garshol.priv.no/blog/329.html source information on Lars Garshol's blog] for brewing notes. Species currently unknown, but most closely related to bayanus / pastorianus / uvarum / arboricolus <ref>[https://www.facebook.com/download/950176781672127/1845813_funits.pdf Accugenix Report from White Labs on WLP 6788. Posted by Eskild Alexander Bergan on Milk The Funk. 6/4/2015.]</ref>.** As noted above, this strain is genetically not related to the other kveik strains, and thus arguably is not actually classified as "kveik" <ref name="garshol_not_kveik" /><ref name="preiss_diagram" />.
* The [https://www.brewingscience.com/product/norwegian-farmhouse "Norwegian Farmhouse Ale" strain from Brewing Science Institute] is likely Muri, but this has not been verified <ref>[https://www.facebook.com/groups/MilkTheFunk/permalink/2491619220866277/ Allen Stone. Milk The Funk Facebook group thread on BSI Norwegian Farmhouse Ale strain. 02/01/2019.]</ref>.

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