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* Generally this method selects for 1-3 strains of yeast with usually a lactic acid bacteria strain.
* The anaerobic nature of this method selects against mold growth.
* This method may not select for wild ''Brettanomyces'' since aerobic growth is limited. This method could possibly be altered to support the growth of ''Brettanomyces'' by allowing for small amounts of oxygen exposure after the initial aerobic step.
==Growing and Testing Without Plating==