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Brettanomyces Propagation Experiment

5 bytes removed, 11:33, 6 November 2015
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Cell counts and pH readings were taken every 24 hours until all treatments showed no or little increase in cell count. Cell counts were done with a hemocytometer. Final gravity for each treatment was recorded at the end of the experiment. The results of this experiment are shown below in figures 1-3.
Because the cell counts were so high and the time to completion was so short, it was thought that these results may be due to the high level of initial cell count. Therefore, another experiment was performed comparing an initial cell count of 30 million cells per mL (low) and 120 million cells per mL (high). This experiment was also The treatments were duplicated. The ''Brett'' inoculum was prepared as described above and each treatment was prepared in 265 mL of wort and incubated on an orbital shaker at 80 RPM and 26°C. Because "life got in the way", cell counts and pH readings were only taken for the first 2 days, and then again on the 6th day. Both treatments in this second experiment resulted in a final gravity of 2°P on day 6. Figures 4-5 show the results of this experiment.
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