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==Methods of Sour Mashing==
Sour mashing procedures fall into the normal all-grain brewday process between completion of saccharification, and any other desired mashing rests, and separation of the wort from the grain. After the normal saccharification rests and a mashout to 170+ F (76.7+ C) to denature enzymes in the mash, the mash is cooled to approximately 120 F (48.9 C). At this point the mash is more or less pasteurized from the hot temperatures of the mash. In order to reintroduce bacteria into the mash it is inoculated with lactic acid bacteria. This may be accomplished by addition of a pure culture of ''[[Lactobacillus]]'' or, more commonly, by the addition of a small amount of unused malt, which has ''Lactobacillus'' in addition to other bacteria and yeasts on the husks (see [[Grain#Mash_and_WortMalted_Barley|bacteria found on malted grain]] for more information).
To help favor the growth of ''Lactobacillus'' over the other unwanted microbes, it is important that the mash be kept anaerobic and incubated warm (ideally 113-120°F/45-48.9°C) throughout the duration of the sour mash. This can be accomplished by purging the headspace with CO2 (some breweries go so far as to bubble CO2 through their mash while it is cooling, and periodically throughout the souring time) and covering with saran wrap at the liquid-air interface to eliminate air contact. The favoring of ''Lactobacillus'' over spoilage microbes can also be supported by dropping the initial sour mash pH by methods such as the addition of acidulated malt, lactic acid (preferably), or starter wort from a ''Lactobacillus'' culture.