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Laboratory Techniques

467 bytes added, 15:26, 27 August 2019
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==Growth Media==
 
===General Notes===
Richard Preiss from [[Escarpment Laboratories]] reported better agar results when limiting autoclave times to 15 minutes for recipes that include copper-containing ingredients <ref>[https://www.facebook.com/groups/MilkTheFunk/permalink/2874530432575152/?comment_id=2874872599207602&reply_comment_id=2874886849206177&comment_tracking=%7B%22tn%22%3A%22R%22%7D Richard Preiss.
Milk The Funk facebook group thread about making agar. 08/27/2019.]</ref>.
 
See also:
* [https://www.facebook.com/groups/MilkTheFunk/permalink/1697319680296239/ MTF tips on autoclaving wort extract based media in such a way that prevents precipitation and darkening.]
* [https://www.facebook.com/groups/MilkTheFunk/permalink/1320512184643659/ MTF tips on what media to use for wild yeast isolation.]
* For techniques on separating yeast strains from themselves and from bacteria, see [[Laboratory_Techniques#Yeast.2FBacteria_Isolation|Yeast/Bacteria Isolation]].
* To detect ''[[Brettanomyces]]'' in a ''[[Saccharomyces]]'' culture, WLN (or WLN along with DBDM) or even something as cheap as YPD can be used because ''Saccharomyces'' will show significant colony growth at 3 days, while ''Brettanomyces'' colonies will grow at 5-10 days <ref>[https://www.facebook.com/groups/MilkTheFunk/permalink/1957870547574483/ Richard Preiss, Justin Amaral, and Lance Shaner. Milk The Funk Facebook thread on detecting Brettanomyces in a Saccharomyces culture. 01/18/2018.]</ref>.
 
Reference on DBDM: https://www.facebook.com/groups/MilkTheFunk/permalink/1805210829507123/?comment_id=1805397166155156&comment_tracking=%7B%22tn%22%3A%22R%22%7D
===''Lactobacillus/Pediococcus''===
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===Misc/Other===
* [https://www.facebook.com/groups/MilkTheFunk/permalink/1697319680296239/ MTF tips on autoclaving wort extract based media in such a way that prevents precipitation and darkening.]
* [https://www.facebook.com/groups/MilkTheFunk/permalink/1320512184643659/ MTF tips on what media to use for wild yeast isolation.]
* For techniques on separating yeast strains from themselves and from bacteria, see [[Laboratory_Techniques#Yeast.2FBacteria_Isolation|Yeast/Bacteria Isolation]].
* To detect ''[[Brettanomyces]]'' in a ''[[Saccharomyces]]'' culture, WLN (or WLN along with DBDM) or even something as cheap as YPD can be used because ''Saccharomyces'' will show significant colony growth at 3 days, while ''Brettanomyces'' colonies will grow at 5-10 days <ref>[https://www.facebook.com/groups/MilkTheFunk/permalink/1957870547574483/ Richard Preiss, Justin Amaral, and Lance Shaner. Milk The Funk Facebook thread on detecting Brettanomyces in a Saccharomyces culture. 01/18/2018.]</ref>.
 
Reference on DBDM: https://www.facebook.com/groups/MilkTheFunk/permalink/1805210829507123/?comment_id=1805397166155156&comment_tracking=%7B%22tn%22%3A%22R%22%7D
==Storage==

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