Changes

Jump to: navigation, search

Wild Yeast Isolation

227 bytes added, 11:41, 16 October 2019
m
no edit summary
==David Thornton's "Yeast Bootcamp"==
This method was developed by David Thornton of [[SouthYeast Labs]] and [https://carolinabauernhaus.com/ Carolina Bauernhaus] for collecting wild ''Saccharomyces'' yeast and lactic acid bacteria. David described his method in detail on [https://www.milkthefunk.live/podcast/2018/6/28/episode-005-bio-prospecting-with-david-thornton-and-keston-helfrich-of-southyeast-labs-and-carolina-bauernhaus the Milk The Funk "The Podcast" episode #005]. Thornton encourages people to alter this process to suit their required results and conditions; for example, developing a different approach that might allow this method to be better suited towards catching ''Brettanomyces''. The following is a summary of this process:
# Start with 75 mL of 6° Plato wort with yeast nutrient in a 250 mL flask. Add the sample (fruit, flower, bee, etc). If it's fruit, squish it up. Set the flask on a shaker or stirplate at 80-90F with a [https://www.facebook.com/groups/MilkTheFunk/permalink/1034954619866085/ foam stopper] (the foam stoppers can be autoclaved/boiled and create an aerobic environment; aluminum foil should work as a substitute) for 12-48 hours. Look for frothing and as soon as that happens put an airlock on the flask, but keep on stirplate to assist fermentation.

Navigation menu