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Laboratory Techniques

305 bytes added, 21:08, 9 March 2020
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'''Yeast Growth Media'''
 
This is a simple non-synthetic yeast growth media shared by Cory Widmayer on Milk The Funk, and is based off of [https://academic.oup.com/femsyr/article/13/1/34/544881 Leite et al. (2013)]. See [https://www.facebook.com/groups/MilkTheFunk/permalink/3328794017148789/ Widmayer's post on MTF] for directions on using this media (requires aeration). This media and method are claimed to grow ''Brettanomyces'' very quickly. Widmayer also provides a synthetic version of the recipe on the MTF post.
 
{| class="wikitable"
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| Glucose or another sugar type || 100 grams
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| Yeast Extract || 90 grams
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| Distilled Water || Fill to 1000 mL
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}
====Misc====
* [https://www.facebook.com/groups/MilkTheFunk/permalink/3328794017148789/ Cory Widmayer's "Yeast Growth Media" and a synthetic yeast media, with an in-depth discussion on nutrient requirements for fast yeast growth (actual growth yields and rate not given).] Formulation is based off of [https://academic.oup.com/femsyr/article/13/1/34/544881 Leite et al. (2013)].
* [https://www.sciencedirect.com/science/article/abs/pii/S0168160519303460 A simple procedure for detecting ''Brettanomyces bruxellensis'' in wine environment by RNA-FISH using a novel probe.]

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