13,591
edits
Changes
added ABD media
NBB media can also be used to help detect/isolate spoilage bacteria. This is a pre-made media by Doehler. More info can be found at [https://www.doehler.com/en/our-portfolio/integrated-solutions/service-solutions/culture-media-for-microbial-detection/dmdr-product-range/nbbr.html NBB Media]. Although ASBC suggests this media its most likely favored by them as their recommendations are generally products sold by Siebel. After a quick discussion with Richard Preiss cheaper media can be used to reach the same goals. Using HLP, WLN w/ cycloheximide and tween 80, and MRS w/ cycloheximide and tween 80 you can achieve the same results as using the array of NBB media available.
'''ABD Media'''
Advanced beer-spoiler detection medium (ABD), which is basically MRS with some of the MRS substituted for beer, has reportedly been shown to be a better growth medium for beer-spoiler LAB which have adapted to the brewing environment and are difficult to grow on other media. This media also has the advantage of inhibiting the growth of other microorganisms that are not beer spoilers. In order to grow some very slow growing strains, microcolony methods using carboxyfluorescein diacetate (CFDA) and species-specific fluorescence ''in situ'' hybridization (FISH) allows detection of slow-growing strains of LAB within 3 days, although the CFDA and FISH approaches require special equipment that might not be available for some QC laboratories <ref>[https://www.ncbi.nlm.nih.gov/pubmed/18070034 Development of detection medium for hard-to-culture beer-spoilage lactic acid bacteria. Suzuki K, Asano S, Iijima K, Kuriyama H, Kitagawa Y. 2008.]</ref><ref>[https://onlinelibrary.wiley.com/doi/pdf/10.1002/j.2050-0416.2011.tb00454.x 125th Anniversary Review: Microbiological Instability of Beer Caused by Spoilage Bacteria. Koji Suzuki. 2011.]</ref>.
{| class="wikitable"
|-
! Chemical !! Usage Amount
|-
| MRS broth (powder) || 2.61 grams
|-
| Sodium acetate || 0.5 grams
|-
| Clycloheximide || 10 milligrams
|-
| Agar || 15 grams
|-
| Beer (presumably lager beer with low IBU) || 1000 milligrams
|-
| Final pH || 5.0
|-
|}
===''Saccharomyces''===